DARC antibody (N-Term)

Catalog No. ABIN2465620

This Goat Polyclonal antibody specifically detects DARC in WB, ELISA, IHC and FACS. It exhibits reactivity toward Human.

Quick Overview for DARC antibody (N-Term) (ABIN2465620)

Target: DARC (Duffy Blood Group, Chemokine Receptor (DARC))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This DARC antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)

Product Details anti-DARC Antibody

Binding Specificity: N-Term

Purification: Duffy antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Duffy antibody was raised against a 19 amino acid synthetic peptide near the N-Terminus of Duffy.

Application Details

Application Notes: ELISA: Antibody detection limit dilution 1:16,000. Western Blot: Approximately 35 kDa band observed in human kidney and human liver lysates (calculated MW of 35.6 kDa according to NP_002027.2). Recommended concentration: 0.1-0.3 μg/mL. Immunohistochemistry: In paraffin embedded human kidney, stains epithelial cells of renal tubules and endothelial cells of vessels. The granular staining is compatible with the endoplasmic reticulum association of the protein. This antibody has also been successfully used on Huamn lung showing endothelial staining. Recommended concentration, 3 μg/mL. FACS: A customer has reported successful staining of HEK293S-TetR transfected with DARC receptor at a dilution of 1/1000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20 °C.

Target Details for DARC

Target: DARC (Duffy Blood Group, Chemokine Receptor (DARC))

Gene ID: 2532

NCBI Accession: NP_002027

UniProt: Q16570